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. 1987 Nov;56(5):539–544. doi: 10.1038/bjc.1987.239

Preferentially expressed genes in stomach adenocarcinoma cells.

T Shiosaka 1, Y Tanaka 1, Y Kobayashi 1
PMCID: PMC2001899  PMID: 3426917

Abstract

cDNA clones complementary to mRNA of neoplastic cells of human stomach tissue were used to examine quantitative changes in the mRNA levels of specific genes in neoplastic cells. Poly(A)+ RNA from poorly differentiated adenocarcinoma cells of a female patient with stomach cancer was used for construction of a complementary DNA (cDNA) library. Screening of the 18,000 colonies utilizing 32P-cDNAs derived from normal human tissue and stomach carcinoma tissue samples was used to select clones likely to represent sequences preferentially expressed in stomach carcinoma cells. Twenty-six recombinants were initially selected and further analysis of these clones indicated that eight (4-3D, 9-2D, 9-4G, 29-1G, 29-6F, 37-1B, 115-5A and 52-5F) contain sequences preferentially expressed in stomach carcinoma cells. We have identified the 9-4G, 29-1A, and 29-6F genes which are differentially expressed in human neoplasia.

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