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. 2001 Jun 15;107(12):1599–1606. doi: 10.1172/JCI12432

Figure 2.

Figure 2

(a) Endocytosis of B cell membrane proteins in K5 expressing BJAB. BJAB cells expressing K5 were transfected with 20 μg of an expression vector for a Ha-tagged dynamin transdominant mutant (known to specifically block endocytosis events) or for an expression vector for the Ha-tagged wild-type dynamin (as control). Forty-eight hours after transfection, cells were incubated with mAb directed against either MHC-I, B7-2, ICAM-1, or CD58 and bound mAb’s were revealed by incubation with an R-phycoerythrin–conjugated goat anti-mouse Ab. Cells were then fixed, incubated in presence of saponin with a fluorescein-conjugated mAb directed against the Ha tag, and analyzed by flow cytometry. Results represent the cell surface expression of MHC-I, B7-2, ICAM-1, and CD58 proteins in the dynamin-transfected cells. (b) BJAB cells expressing K5 or control cells were incubated for 12 hours in presence of 50 mM ammonium chloride. Cells were then fixed, incubated with mAb’s against B7-2 and ICAM-1 in the presence of saponin, and analyzed by flow cytometry.