Fig. 4.

a. BMSCs from myostatin-deficient mice cultured in osteogenic medium and stained for alkaline phosphatase (ALP) and Von Kossa to detect mineralized nodule formation show similar staining in the presence and absence of recombinant myostatin. Different columns of dishes represent three sample replicates. 4b. Gene expression of Mstn^-/- adherent stromal cells after 3 days of cyclic hydrostatic compressive stress stimulation, consisting of 1 atmosphere at 0.5 Hz for 3 h/day. 24 h prior to testing, and between tests, cultures were incubated with 0, 10, or 100 ng/ml myostatin. Data (mean+/-SEM) are shown relative to unstressed controls which had been incubated with the same amount of myostatin. Loading increased the expression of osteogenic factors, but the addition of myostatin mitigated this trend of upregulation.