Table 1.
Regulation of CD40L expression on human vascular SMC, EC, or macrophages (MØ) by proinflammatory cytokines
| Cell type | CD40L mean fluorescence intensity
|
|||
|---|---|---|---|---|
| None | IL-1β | TNF-α | IFN-γ | |
| SMC | 38.3 | 128.1 | 72.4 | 62.2 |
| EC | 5.8 | 18.6 | 17.7 | 15.5 |
| MØ | 6.7 | 12.5 | 12.4 | 12.1 |
Cells cultured under quiescent conditions (serum-free media) were incubated 24 h without cytokine (None), or with 10 ng/ml IL-1β, 50 ng/ml TNF-α, or 1000 units/ml IFN-γ, and analyzed for CD40L expression by flow cytometry. Similar results were obtained from three different experiments.