Table 2.
Regulation of B7.2 expression on human IB4 cells coincubated with human vascular SMC, EC, or human macrophages (MØ) by CD40–CD40L interaction
| IB4 cocultured cell type | B7.2 mean fluorescence intensity
|
|
|---|---|---|
| − | + | |
| SMC | 672.6 | 208.5 |
| EC | 482.5 | 199.5 |
| MØ | 456.7 | 232.1 |
| None | 195.8 | 178.5 |
IB4 cells (0.5 × 106/ml) were coincubated for 48 h with confluent cultures of human SMC, EC, or human MØ in serum-containing media, in the absence (−) or presence (+) of anti-CD40L mAb (10 μg/ml). Nonadherent cells were harvested and CD19+ cells were explored for B7.2 expression by flow cytometry analysis. IB4 cells alone were used as control (None).