Figure 5.
PCR after serial dilutions of cDNA shows a relative decrease in amounts of the targeted RSV M2 mRNA. PCR products from the RSV M2 (○, •) and P (□, ▪) mRNAs were separated on agarose gels and stained with ethidium bromide. Quantitation of bands plotted in the semilogarithmic plot shown was by densitometry. RNA was from either untreated infected cells (○, □) or infected cells treated at 2 h postinfection with 3.3 μM spA4-antiRSV3′-3′T/(8281–8299) (•, ▪). RNA was harvested at 8 h postinfection. (Inset) An image of the PCR products from untreated and spA4-antiRSV3′-3′T/(8281–8299)-treated cultures in ethidium bromide-stained gels.
