Table 2.
Oligodeoxyribonucleotide primers used for PCR and RT reactions
| Target | Sequence | Annealing temp., °C | Product size, bp |
|---|---|---|---|
| G3PDH(+) | 5′-AAATCCCATCACCATCTTC-3′ | 56 | 762 |
| G3PDH(−) | 5′-CACCACCCTGTTGCTGTAG-3′ | ||
| RSV (M2+) | 5′-AAACAATCAGCATGTGTTG-3′ | 46 | 587 |
| RSV (M2−) | 5′-AATGTAACGATGTGGTGAG-3′ | ||
| RSV (N+) | 5′-ACAACACATCGTCAAG-3′ | 46 | 577 |
| RSV (N−) | 5′-ATTCATAAACCTCAAC-3′ | ||
| RSV (P+) | 5′-AAACAACAGGGCTACTA-3′ | 46 | 575 |
| RSV (P−) | 5′-TTTCACTTTCCTCATTC-3′ | ||
| RSV (L−) | 5′-GAGCTTTATTAGCAGCATC-3′ | RT primer | |
The target genes are indicated on the left, followed by the target sequence and the optimal annealing temperature as determined by the program oligo (version 4.1, National Biosciences, Plymouth, MN). G3PDH, glyceraldehyde-3-phosphate dehydrogenase.