Figure 3.

In vitro induction of immune responses by dendritic cell fractions from retroviral vector-immunized mice. Five mice were immunized with retroviral vectors encoding N2-IIIBenv by three injections, and their B + T cells, dendritic cells, and macrophage fractions were prepared as described in Materials and Methods. Five littermates were processed in an analogous manner. Each of the reconstituted splenocyte cultures contains B + T cells (4 × 10⁷), dendritic cells (1 × 10⁷), and macrophage (6 × 10⁶) fractions and irradiated stimulators (1/50 of total responders). + and −, Fractions from immunized or naive mice, respectively. Each set of bars represents net CTL lysis at effector-to-target cell ratios from 100, 30, 10, 3, and 1.