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. 1997 Mar 4;94(5):1949–1954. doi: 10.1073/pnas.94.5.1949

Figure 2.

Figure 2

Activation of the MCSFR proximal promoter by CBFA2B and ETV6 or ETV6/CBFA2 with or without C/EBPa. (A) ETV6 has no detectable effect on reporter gene by itself. Together CBFA2B and C/EBPa synergistically activate the reporter gene approximately 80-fold. This synergistic activation is inhibited by the addition of either ETV6 or ETV6/CBFA2. (B) In the absence of C/EBPa, CBFA2B only activates the reporter gene 5-fold above background. The cotransfection of ETV6 or ETV6/CBFA2 in the absence of C/EBPa slightly increases reporter gene activation. (C) C/EBPa activates the MCSFR promoter about 15-fold in the absence of cotransfected CBFA2. Both ETV6 and ETV6/CBFA2 inhibit the activation of the promoter by C/EBPa, even when CBFA2 is not included in the cotransfection assay. Note also that the cotransfection of ETV6, together with ETV6/CBFA2 in the presence of C/EBPa, inhibits the promoter to a relative activation of 0.035-fold.