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. 1997 Mar 4;94(5):1949–1954. doi: 10.1073/pnas.94.5.1949

Figure 3.

Figure 3

Inhibition of activation of the MCSFR promoter by increasing amounts of ETV6 or ETV6/CBFA2 plasmid. (Upper) Shows that increasing amounts of either ETV6 or ETV6/CBFA2 increases the level of reporter gene inhibition. It also shows that the removal of the HLH domain of either ETV6 or ETV6/CBFA2 results in a decrease in the ability of the respective constructs to inhibit promoter activation. (Lower) Results of cotransfecting the CBFA2 splice varient CBFA2A or the mutant ETV6/CBFA2 construct lacking the activation domain into the cotransfection system. The del AD ETV6/CBFA2 is able to inhibit reporter gene expression more than the full-length ETV6/CBFA2. CBFA2A, lacking the activation domain of CBFA2, is also able to decrease the synergistic activation of the promoter by CBFA2B and C/EBPa.