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Activation of the hexamer reporter, a 29-bp region of the MCSFR proximal promoter containing the CBF and C/EBPa binding sites. (A) Addition of both CBFA2B and C/EBPa results in the synergistic activation of the hexamer reporter. Cotransfection of either ETV6 or ETV6/CBFA2 results in the inhibition of reporter gene activation. (B) Inhibition by ETV6 and ETV6/CBFA2 is not detectable in the absence of C/EBPa.
