Abstract
Cellular and vascular proliferation in the perisinusoidal area of the acinar zone III of the liver preceded the formation of septal fibrosis in rats repeatedly treated with intraperitoneal injections of pig serum. Deposition of type I, III, IV and V collagens and increased amounts of fibronectin and laminin were also observed. Immunochemical staining for actin, elastin and desmin better disclosed the rich vascularization of the fibrous septa. Hypertrophy and hyperplasia of sinusoidal fat-storing cells point to the main role of such cells in the process of septum formation, but other connective tissue cells, such as mast-cells, were also present. These changes seemed to result from stimulation of the sinusoidal macrophage/fat-storing-cell system by foreign material present in the pig serum. The model may be useful for further studies on pathogenesis of septal fibrosis of the liver.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andrade Z. A. Evolution and involution of hepatosplenic schistosomiasis. Mem Inst Oswaldo Cruz. 1989 Oct;84 (Suppl 1):58–75. doi: 10.1590/s0074-02761989000500007. [DOI] [PubMed] [Google Scholar]
- Andrade Z. A., Grimaud J. A. Morphology of chronic collagen resorption. A study on the late stages of schistosomal granuloma involution. Am J Pathol. 1988 Aug;132(2):389–399. [PMC free article] [PubMed] [Google Scholar]
- Arenson D. M., Friedman S. L., Bissell D. M. Formation of extracellular matrix in normal rat liver: lipocytes as a major source of proteoglycan. Gastroenterology. 1988 Aug;95(2):441–447. doi: 10.1016/0016-5085(88)90502-1. [DOI] [PubMed] [Google Scholar]
- Ballardini G., Faccani A., Fallani M., Berti S., Vasi V., Castaldini C., Biagini G., Garbisa S., Bianchi F. B. Sequential behaviour of extracellular matrix glycoproteins in an experimental model of hepatic fibrosis. Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(4):317–324. doi: 10.1007/BF02912109. [DOI] [PubMed] [Google Scholar]
- CAMPBELL J. A. The effects of adrenalectomy in experimental non-dietary cirrhosis. Br J Exp Pathol. 1963 Feb;44:66–71. [PMC free article] [PubMed] [Google Scholar]
- Cenacchi G., Ballardini G., Badiali De Giorgi L., Busachi C. A., Del Rosso M., Bianchi F. B., Biagini G., Laschi R. Relationship between connective tissue cells and fibronectin in a sequential model of experimental hepatic fibrosis. Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;43(1):75–84. doi: 10.1007/BF02932946. [DOI] [PubMed] [Google Scholar]
- FENNELL R. H., Jr, SANTAMARIA A. Anaphylaxis in the rat. Immunohistochemical and histochemical studies of the liver. Am J Pathol. 1962 Nov;41:521–534. [PMC free article] [PubMed] [Google Scholar]
- Friedman S. L., Roll F. J., Boyles J., Bissell D. M. Hepatic lipocytes: the principal collagen-producing cells of normal rat liver. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8681–8685. doi: 10.1073/pnas.82.24.8681. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fujiwara K., Ogata I., Ohta Y., Hayashi S., Mishiro S., Takatsuki K., Sato Y., Yamada S., Hirata K., Oka H. Decreased collagen accumulation by a prolyl hydroxylase inhibitor in pig serum-induced fibrotic rat liver. Hepatology. 1988 Jul-Aug;8(4):804–807. doi: 10.1002/hep.1840080418. [DOI] [PubMed] [Google Scholar]
- Gressner A. M., Zerbe O. Kupffer cell-mediated induction of synthesis and secretion of proteoglycans by rat liver fat-storing cells in culture. J Hepatol. 1987 Dec;5(3):299–310. doi: 10.1016/s0168-8278(87)80036-3. [DOI] [PubMed] [Google Scholar]
- Levison D. A., Kingham J. G., Dawson A. M., Stansfeld A. G. Slow cirrhosis--or no cirrhosis? a lesion causing benign intrahepatic portal hypertension. J Pathol. 1982 Jul;137(3):253–272. doi: 10.1002/path.1711370309. [DOI] [PubMed] [Google Scholar]
- McGee J. O., Patrick R. S. The role of perisinusoidal cells in hepatic fibrogenesis. An electron microscopic study of acute carbon tetrachloride liver injury. Lab Invest. 1972 Apr;26(4):429–440. [PubMed] [Google Scholar]
- Minato Y., Hasumura Y., Takeuchi J. The role of fat-storing cells in Disse space fibrogenesis in alcoholic liver disease. Hepatology. 1983 Jul-Aug;3(4):559–566. doi: 10.1002/hep.1840030414. [DOI] [PubMed] [Google Scholar]
- Paronetto F., Popper H. Chronic liver injury induced by immunologic reactions. Cirrhosis following immunization with heterologous sera. Am J Pathol. 1966 Dec;49(6):1087–1101. [PMC free article] [PubMed] [Google Scholar]
- Popper H., Uenfriend S. Hepatic fibrosis. Correlation of biochemical and morphologic investigations. Am J Med. 1970 Nov;49:707–721. doi: 10.1016/s0002-9343(70)80135-8. [DOI] [PubMed] [Google Scholar]
- Rubin E., Hutterer F., Popper H. Experimental hepatic fibrosis without hepatocellular regeneration. A kinetic study. Am J Pathol. 1968 Jan;52(1):111–120. [PMC free article] [PubMed] [Google Scholar]
- Sciot R., Staessen D., Van Damme B., Van Steenbergen W., Fevery J., De Groote J., Desmet V. J. Incomplete septal cirrhosis: histopathological aspects. Histopathology. 1988 Dec;13(6):593–603. doi: 10.1111/j.1365-2559.1988.tb02091.x. [DOI] [PubMed] [Google Scholar]
- Stenger R. J. Hepatic sinusoids in carbon tetrachloride-induced cirrhosis. An electron microscopic study. Arch Pathol. 1966 May;81(5):439–447. [PubMed] [Google Scholar]
- Tanaka Y., Mak K. M., Lieber C. S. Immunohistochemical detection of proliferating lipocytes in regenerating rat liver. J Pathol. 1990 Feb;160(2):129–134. doi: 10.1002/path.1711600206. [DOI] [PubMed] [Google Scholar]
- Tsukamoto H., Matsuoka M., French S. W. Experimental models of hepatic fibrosis: a review. Semin Liver Dis. 1990 Feb;10(1):56–65. doi: 10.1055/s-2008-1040457. [DOI] [PubMed] [Google Scholar]