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. 1997 Mar 4;94(5):1955–1960. doi: 10.1073/pnas.94.5.1955

Figure 1.

Figure 1

R4A, 95, and 52b3 binding by ELISA to various phage clones. Microtiter 96-well plates were coated with 1 μg/ml purified antibody, and 2.5 × 1010 phage were added to each well, followed by biotinylated antibody to M13 phage, and streptavidin-alkaline phosphatase. OD after addition of substrate was monitored at 405 nm. Shaded boxes (left) are R4A selected clones, and open boxes (right) are 52b3 selected clones. (A) R4A reactivity with R4A and 52b3 selected clones. (B) 95 reactivity with R4A and 52b3 selected clones. (C) 52b3 reactivity with R4A and 52b3 selected clones.