Figure 1. Structure of MHC-lacZ fusion and reporter gene constructs.
Constructs A, B, C and G were made by fusing the lacZ gene of pOX3 (Raghaven et al., 1986) in frame to the Mhc coding region. Constructs D, E and F were derived from pDM30 (Mismer and Rubin, 1987), which contains the Drosophila hsp70 minimal promoter linked to a lacZ reporter. The parental lacZ plasmids also provide a polyadenylation signal. All constructs were inserted into a P element plasmid vector prior to germline transformation. Details are given in Experimental Procedures.