Skip to main content
. Author manuscript; available in PMC: 2007 Oct 9.
Published in final edited form as: J Mol Cell Cardiol. 2006 Sep 28;41(5):834–844. doi: 10.1016/j.yjmcc.2006.08.008

Fig. 2.

Fig. 2

Activation of CXCR4 decreases CM contractility. Adult rat CM were exposed to CXCL12 (125 ng/ml) for either 5, 10 or 60 min prior to a stepwise Ca2+ challenge (A, B) or to increasing ISO concentrations after a 5-min exposure to CXCL12 (C). In both experiments, control CM were exposed to diluent only. The average percent increase in shortening (A) and average peak Ca2+ transients (B) during the Ca2+ challenge were significantly lower in CM exposed for either 5 or 10 min to CXCL12. No difference in shortening or Ca2+ transient was detected in CM exposed for 60 min to CXCL12 (A and B). Percent increase in fractional shortening in the CM is shown as a function of increasing [ISO] (0.001–10 μM) (C). CXCL12 exposed CM demonstrated a significant decrease in shortening response. ISO experiments were performed in triplicate.