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. Author manuscript; available in PMC: 2007 Oct 9.
Published in final edited form as: FEMS Microbiol Ecol. 2006 Feb;55(2):248–261. doi: 10.1111/j.1574-6941.2005.00022.x

Fig. 5.

Fig. 5

Inhibition of the dechlorination of 2,3,4,5-tetrachlorobiphenyl (CB) in sediment-free subcultures by 2-bromoethanesulfonic acid (BESA) (a) and the denaturing gradient gel electrophoresis pattern showing the bacterial communities in the subcultures on Day 194 (b). Black triangles show subcultures to which no BESA was added and white triangles show subcultures amended with BESA. Lane D contains DNA for Dehalococcoides ethenogenes as a marker; lanes 1–3 show the dechlorinating sediment-free cultures to which no BESA was added and lanes 4–6 show the sediment-free cultures to which BESA was added that were not capable of dechlorination. The arrows point to the bands corresponding to the Dehalococcoides-like population, which were excised and sequenced.