Figure 1.

Stable transfection and overexpression of calbindin-D_28k (CB28) in RIN 1046 cells. (A) pREP-CB28 construct. (B) Western blot analysis of clones transfected with either vector alone (V9, V10, and V11) or pREP-CB28 (C2, C10, C11, C13, and C5). The results shown are representative of results obtained in five separate experiments. (The split band in clone C5 is likely an artifact of proteolysis and was not otherwise seen in several other Western blots of calbindin expression in C5 and other clones.)