Figure 5.

Transient transfection of vector clones and calbindin-D_28k-overexpressing clones C2 and C10 with rInsI promoter–CAT gene constructs. (Top) Schematic representation of the rInsI gene 5′ flanking DNA. (Middle) Enhancement of CAT activity using −345/+1 or 5 FF in calbindin-transfected cells. (Bottom) Graphic representation of CAT activity (each bar represents the mean ± SEM of three to five independent transfections). CAT activity using −345/+1 or 5 FF in the calbindin-overexpressing clones C2 or C10 was significantly different from CAT activity in vector-transfected cells (P < 0.05).