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. Author manuscript; available in PMC: 2007 Oct 9.
Published in final edited form as: Dev Dyn. 2007 Aug;236(8):2225–2234. doi: 10.1002/dvdy.21225

Fig. 4.

Fig. 4

Intracellular localization of endogenous leucine-rich repeat containing protein 10 (LRRC10) in cardiomyocytes. A–C: Immunostaining of primary cultured cardiomyocytes. Neonatal mouse cardiomyocytes grown on cover slips for 2 days (A,B) or 7 days (C) were fixed in 100% methanol. Fixed cells were incubated with anti-LRRC10 and MF20 or cardiac α-actinin antibodies followed by secondary antibodies coupled to Alexa 488 or 546. D: The striated expression pattern of LRRC10 in adult heart sections. Frozen mouse heart sections were immunostained with LRRC10 antibodies followed by anti-rabbit immunoglobulin G–Alexa 488. Arrowheads indicate cardiomyocytes that are MF20-positive. Arrow points the striated pattern of LRRC10 in neonatal cardiomyocytes.