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. Author manuscript; available in PMC: 2007 Oct 9.
Published in final edited form as: Dev Dyn. 2007 Aug;236(8):2225–2234. doi: 10.1002/dvdy.21225

Fig. 6.

Fig. 6

Examination of leucine-rich repeat containing protein 10 (LRRC10) localization using skinned adult cardiomyocyte. A–C: Intact or skinned cardiomyocytes isolated from adult rats were co-immunostained with anti-LRRC10 and wheat germ agglutinin (WGA) -conjugated with Alexa Flour 488, T-tubule marker (A) or RyR, sarcoplasmic reticulum marker (B) or cardiac α-actinin, sarcomeric Z-line marker (C) antibodies. D: C2C12 myoblasts transfected with Lrrc10-pcDNA3.1-myc (left column) were differentiated into myotubes (right column) and immunostained with anti-LRRC10 antibody. E: C2C12 myotubes were co-immunostained with anti-myc and α-actinin (sarcomeric Z-line marker) or myomesin (sarcomeric M-line marker) antibodies and shown at a higher magnification. Cells were incubated with the secondary antibodies coupled to Alexa 488 or 546 and examined under confocal microscope. Nuclei were visualized by Hoechst staining.