Fig. 7.

Electron micrographs showing immunogold labeling of leucine-rich repeat containing protein 10 (LRRC10). Adult mouse heart tissue was incubated with an affinity-purified LRRC10 antibodies followed by gold-labeled secondary anti-rabbit antibodies. A,B: Black dots represent the LRRC10 localization at the diad region where the sarcoplasmic reticulum (SR) interacts with the T-tubule. C,D: Negative controls were performed with nonspecific rabbit immunoglobulin G instead of anit-LRRC10 antibodies followed by gold labeled secondary antibodies. The representative pictures are shown from three independent experiments. z, Z-line; mt, mitochondria. Scale bar = 1 μm.