Fig. 4.

Recombination activities of Flp variants with substitutions at Trp-330. Recombination was assayed using the deletion reaction schematically indicated at the top. The substrate plasmid is the same as that employed in the in vivo assays depicted in Fig. 9 and 12. Reaction mixtures digested with SphI were analyzed by electrophoresis in agarose gels. ‘P’ and ‘R1’ refer to the parent plasmid and the larger deletion circle (or their linear forms in the ethidium bromide stained gel), respectively. ‘R2’ indicates the smaller deletion circle.