Figure 3.
Rab-7 is required for normal chemotaxis. (A) Head region of a rab-7∷gfp-expressing transgenic worm visualized using an FITC filter (left panel) showing expression of GFP. The same worm was dye-filled with DiI and visualized under a rhodamine filter (middle panel) to show the amphid neurons. The two photos were merged (right panel) to show that rab-7 is also expressed in the amphid neurons. (B) Chemotaxis assay of wild-type worms on control RNAi or rab-7 RNAi. Aliquots (1 μl) of benzaldehyde (1:200 diluted), isoamyl alcohol (1:100) or pyrazine (10 mg/ml) were used for the assay. DiI, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine iodade; FITC, fluorescent isothiocyanate; GFP, green fluorescent protein; RNAi, RNA interference.