Figure 2.

Overexpression of SnRK1A Relieves the Sugar Repression of SRC.

Rice embryos were cotransfected with effector and reporter plasmids and incubated with 100 mM glucose (+G) or without glucose (−G), and their luciferase activities were assayed. The luciferase activity in rice embryos bombarded with the reporter construct only and in the +G condition was assigned a value of 1×, and other values were calculated relative to this value. Error bars indicate the se for three replicate experiments.

(A) The effector contains the Ubi-SnRK1A, Ubi-MAPK4, or Ubi-CDPK1 fusion gene.

(B) Luciferase activity of a reporter containing the SRC-35S mp-Luc fusion gene. SRC contains a duplicated TA box.

(C) Luciferase activity of a reporter containing the 6xTA-35S mp-Luc fusion gene.