Figure 4.

SnRK1 Contains Two Functional Domains That Regulate SRC.

Rice embryos were cotransfected with plasmids and incubated with 100 mM glucose (+G) or without glucose (−G), and their luciferase activities were assayed.

(A) The effector contains the Ubi promoter fused upstream of the full-length cDNA or the kinase domain (K) or regulatory domain (R) of SnRK1A and SnRK1B. The reporter contains the SRC-35S mp-Luc fusion gene.

(B) and (C) Luciferase activity in rice embryos. The luciferase activity in rice embryos bombarded with the SRC-35S mp-Luc construct only and in the +G condition was assigned a value of 1×, and other values were calculated relative to this value. Error bars indicate the se for three replicate experiments.