Table 1.
Primers Used for Plasmid Construction, cDNA Cloning, PCR, RT-PCR, and Real-Time RT-PCR
| Primer | Sequence | Use |
|---|---|---|
| SnRK1A-1F | 5′-ATTGGATCCGAGGGAGCTGGCAGAGATG-3′ | SnRK1A and pUSnf1A(K) |
| SnRK1A-4R | 5′-ATTGGATCCTTAAAGGACTCTCAGCT-3′ | SnRK1A and pUSnf1A(R) |
| SnRK1B-1F | 5′-ATTGGATCCGCATCCATCCTGGTCACTGTGAAA-3′ | SnRK1B and pUSnf1B(K) |
| SnRK1B-4R | 5′-GCGGGATCCGTGTCCTTTATTCATTATTGCAGT-3′ | SnRK1B and pUSnf1B(R) |
| SnRK1A-2R | 5′-ATTGGATCCTTAGTCAGGAGGTGGCACAG-3′ | pUSnf1A(K) |
| SnRK1A-3F | 5′-ATTGGATCCATGGTGCCACCTCCTGACA-3′ | pUSnf1A(R) |
| SnRK1B-2R | 5′-ATTGGATCCCTAGTAACGAGGAAGGCGAATCTG-3′ | pUSnf1B(K) |
| SnRK1B-3F | 5′-ATTGGATCCATGACAGCACAGCAAGCCAAAATG-3′ | pUSnf1B(R) |
| S1-F | 5′-CCGCTCGAGTCTTGTCTTCTTGCTTCCTTTGGTACTTGCC-3′ | pS1GUS |
| S1-R | 5′-CGCGGATCCCGGGTGGTGGATCTCGCACTCTCGC-3′ | pS1GUS |
| 1A1 | 5′-GAATCTTCTCTCGCTCAAGTA-3′ | SnRK1A T-DNA insertion |
| 1A2 | 5′-TCACATTGTGGGTTGATTTCT-3′ | SnRK1A T-DNA insertion |
| 1A4 | 5′-GGGTAACCAATCTGAGT-3′ | SnRK1A 3′ end RT-PCR |
| 1A5 | 5′-CCGCCGCGCGCCGGAGGTCAGTA-3′ | SnRK1A 5′ end RT-PCR |
| 1A6 | 5′-TTACCAGATATCACCTCAGGTGCT-3′ | SnRK1A 5′ end RT-PCR |
| 1A7 | 5′-GCGACAGTTGCCTACTATTTAC-3′ | SnRK1A poly(A) tail and SnRK1A 3′ end RT-PCR |
| 1A11 | 5′-TTCTTAAAGCTCTGCAAGAGC-3′ | SnRK1A 3′ end RT-PCR |
| Q1A5 | 5′-TTATGCCGTTGTCTGCTTC-3′ | SnRK1A real-time RT-PCR |
| Q1A3 | 5′-CCTCTAACGTCTACACACTCCAG-3′ | SnRK1A real-time RT-PCR |
| 35SLB | 5′-GGAATTCAATTCGGCGTTAAT-3′ | SnRK1A KD RT-PCR |
| 1B1 | 5′-TGGCATGTGATTTTAACCTGA-3′ | SnRK1B T-DNA insertion |
| 1B2 | 5′-TATAAGGTCTGGTTAGCCTAC-3′ | SnRK1B T-DNA insertion |
| 1B3 | 5′-CCCCGTGTTGGCTTCAGTG-3′ | SnRK1B 3′ end RT-PCR and real-time RT-PCR |
| 1B4 | 5′-TGATCATACGACTACATTTGTTG-3′ | SnRK1B 3′ end RT-PCR and real-time RT-PCR |
| 1B7 | 5′-TTTTTTAAAGACAAGCTGCGGGAGT-3′ | SnRK1B RT-PCR |
| 1B8 | 5′-GTAACGAGGAAGGCGAATCTG-3′ | SnRK1B RT-PCR |
| S1RTF | 5′-ATGGACGGACATGAGC-3′ | MYBS1 RT-PCR and real-time RT-PCR |
| S1RTR | 5′-GCTTTCACCGGGTGTA-3′ | MYBS1 RT-PCR and real-time RT-PCR |
| 3RT25A | 5′-GTAGGCAGGCTCTCTAGCCTCTAGG-3′ | αAmy3 RT-PCR and real-time RT-PCR |
| 3RT-R | 5′-AACCTGACATTATATATTGCACC-3′ | αAmy3 RT-PCR and real-time RT-PCR |
| Act15 | 5′-CTGATGGACAGGTTATCACC-3′ | Act1 RT-PCR |
| Act13 | 5′-CAGGTAGCAATAGGTATTACAG-3′ | Act1 RT-PCR |
| RB | 5′-AACTCATGGCGATCTCTTACC-3′ | SnRK1A and SnRK1B T-DNA insertion |
| Oligo(dT) anchor | 5′-GCGAGCTCCGCGGCCGCGTTTTTTTTTTTT-3′ | SnRK1A poly(A) tail RT-PCR |
| 18SF | 5′-CCTATCAACTTTCGATGGTAGGATA-3′ | 18S rRNA real-time RT-PCR |
| 18SR | 5′-CGTTAAGGGATTTAGATTGTACTCATT-3′ | 18S rRNA real-time RT-PCR |
Underlined areas indicate restriction sites: CTCGAG, XhoI site; GGATCC, BamHI site.