Figure 2.

RNAi and Overexpression Constructs for At5g13220 Differentially Affect Sensitivity to MeJA.

(A) Seeds were germinated on agar containing either no MeJA (left plate) or MeJA (25 μM; right plate) for 7 d, at which time they were photographed. Wild-type and a coi1-1 segregating seeds were used as controls for MeJA sensitivity.

(B) Dry weight of aerial tissues for plants grown in the absence or presence of MeJA (25 μM). Plants were grown for 21 d prior to harvest (n = 12; error bars indicate sd). Ri7, RNAi-7; Ri9, RNAi-9; OE4A, overexpression line 4A; OE4B, overexpression line 4B.

(C) Transcript levels relative to EIF4A1 for At5g13220 (all transcript forms) in wild-type and transgenic plants. Quantitative PCR was used to measure relative transcript abundance in 4-week-old soil-grown plants (n = 3; error bars indicate sd). Transcript abundance was not normalized to EIF4A1 transcript abundance.