Figure 3.

Overexpression of At5g13220.3 Affects the Activity of JA Biosynthesis Enzymes, AOS, OPR3, and VSP2 Transcript Levels, and JA Synthesis in Response to Wounding.

(A) Extracts of resting leaves were assayed for their ability to oxygenate the jasmonate precursor α-linolenic acid (18:3) in vitro (n = 4; error bars indicate sd).

(B) Relative transcript levels in resting leaves for two JA biosynthesis genes (AOS and OPR3) and for the VSP2 gene (n = 3; error bars indicate sd).

(C) JA measured in leaves before and after wounding. The wound-induced level of JA at 1.5 h was significantly lower in plants overexpresssing the At5g13320.3 transcript than in wild-type plants (*P = 0.035; n = 3; error bars indicate sd). FWT, fresh weight.