Table 1.
List of Genes Differentially Expressed between aos and Col-0
| Wounding (60 min)
|
||||||
|---|---|---|---|---|---|---|
| Locus | Description | aos:Col-0a | Pb | Col-0:ctla | aos:ctla | Pc |
| At2g24850 | Aminotransferase, putative | 0.126 | 5.1E-05 | 12.58 | 2.60 | 0.0312 |
| At5g42650 | Allene oxide synthase (AOS) | 0.151 | 4.2E-05 | 5.22 | 0.63 | 0.0112 |
| At5g47240 | MutT/nudix family protein | 0.171 | 3.6E-04 | 10.30 | 1.26 | 0.0028 |
| At1g74950 | Expressed protein | 0.178 | 6.3E-03 | 5.72 | 1.11 | 0.0026 |
| At1g52030 | Myrosinase binding protein | 0.185 | 0.0034 | 3.07 | 0.83 | 0.0094 |
| At5g34831 | Pseudogene, hypothetical protein | 0.194 | 3.6E-05 | 10.33 | 1.04 | 0.0229 |
| At4g24350 | Phosphorylase family protein | 0.217 | 2.1E-05 | 4.63 | 0.85 | 0.0006 |
| At2g38750 | Annexin 4 (ANN4) | 0.235 | 0.0008 | 9.79 | 1.05 | 0.0387 |
| At1g72450 | Expressed protein | 0.249 | 0.0257 | 5.58 | 1.58 | 0.0251 |
| At4g15440 | Hydroperoxide lyase (HPL1) | 0.259 | 5.0E-05 | 4.75 | 1.20 | 0.0159 |
| At1g43160 | AP2 domain–containing protein RAP2.6 | 0.280 | 1.2E-05 | 15.19 | 1.16 | 0.0008 |
| At2g38240 | Oxidoreductase, 2OG-Fe-oxygenase | 0.283 | 0.0004 | 35.72 | 1.13 | 0.0052 |
| At2g43530 | Trypsin inhibitor, putative | 0.305 | 0.0019 | 5.57 | 0.85 | 0.0058 |
| At5g13220 | Expressed protein | 0.307 | 0.0012 | 15.60 | 1.02 | 0.0006 |
| At1g51760 | Indole-3-acetic acid-Ala hydrolase (IAR3) | 0.310 | 0.0070 | 12.57 | 1.63 | 0.0017 |
| At3g16470 | Jacalin lectin | 0.335 | 0.0017 | 3.64 | 0.90 | 0.0069 |
| At1g20510 | 4-Coumarate-CoA ligase | 0.342 | 0.0081 | 3.36 | 1.47 | 0.0492 |
| At2g43550 | Trypsin inhibitor, putative | 0.344 | 0.0009 | 2.71 | 0.61 | 0.0074 |
| At1g66760 | MATE efflux family | 0.359 | 0.0344 | 7.65 | 1.56 | 0.0065 |
| At2g39330 | Jacalin lectin | 0.381 | 0.0359 | 3.02 | 0.84 | 0.0044 |
| At5g53750 | Expressed protein | 0.385 | 0.0167 | 11.13 | 1.10 | 0.0002 |
| At4g10390 | Putative protein kinase | 0.394 | 0.0100 | 16.25 | 1.43 | 0.0111 |
| At5g10300 | Hydrolase, α/β-fold family | 0.395 | 0.0012 | 5.32 | 0.83 | 0.0012 |
| At2g46510 | Basic helix-loop-helix (bHLH) family | 0.409 | 0.0411 | 5.02 | 1.53 | 0.0006 |
| At2g39030 | GCN5-related N-acetyltransferase (GNAT) | 0.417 | 0.0142 | 4.97 | 1.08 | 0.0179 |
| At2g22330 | Cytochrome P450, putative | 0.417 | 0.0006 | 4.85 | 1.03 | 0.0040 |
| At4g16760 | Acyl-CoA oxidase (ACX1) | 0.418 | 0.0007 | 2.50 | 1.12 | 0.0334 |
| At1g52890 | No apical meristem (NAM) family | 0.423 | 0.0048 | 5.69 | 0.97 | 0.0015 |
| At4g08170 | Inositol 1,3,4-trisphosphate 5/6-kinase | 0.426 | 0.0056 | 11.33 | 1.08 | 0.0006 |
| At1g32640 | Basic helix-loop-helix protein (RAP-1) | 0.435 | 0.0135 | 3.56 | 1.16 | 0.0358 |
| At5g54170 | Expressed protein | 0.438 | 0.0356 | 11.70 | 1.32 | 0.0003 |
| At4g39980 | Dehydrodeoxyphosphoheptonate aldolase | 0.440 | 0.0015 | 4.98 | 0.99 | 0.0002 |
| At2g20340 | Tyrosine decarboxylase, putative | 0.456 | 0.0087 | 8.12 | 0.88 | 0.0003 |
| At2g32150 | Haloacid dehalogenase-like hydrolase | 0.474 | 0.0016 | 2.76 | 0.90 | 0.0077 |
| At2g29440 | Glutathione S-transferase, putative | 0.478 | 0.0219 | 14.73 | 1.97 | 0.0069 |
Col-0, Columbia-0. The list shows genes that were expressed at a lower level (ratio ≤ 0.5, P < 0.05) in the resting (control [ctl]) leaves of aos than in resting leaves of Col-0 and that were wound-inducible in Col-0 but significantly less or not wound-inducible in aos (P < 0.05). cDNAs for genes listed in boldface were overexpressed in plants for MeJA sensitivity experiments.
Expression ratio.
P values (Student's t test, one-sample hypothesis) were calculated by comparing aos:Col-0 ratios (n = 4) with the expected value of 1, indicating no change in gene expression.
P values (Student's t test, two-sample hypothesis) were calculated by comparing expression ratios from Col-0:ctl (n = 3) with expression ratios from aos:ctl (n = 3) in wounding experiments.