Table 1.
Yeast Three-Hybrid Assays Testing BEL1 and MADS Box Protein Interactions
| −WLAH + 3AT
|
||||||||
|---|---|---|---|---|---|---|---|---|
| GAL4-AD | pTFT1 | GAL4-BD | +1 mM | +2.5 mM | +5 mM | +10 mM | +15 mM | +20 mM |
| BEL1 | SEP3 | AG | / | / | + + | + + | + + | + + |
| Empty | SEP3 | AG | / | / | + | + | + | + |
| BEL1 | empty | AG | − | / | − | − | − | − |
| BEL1 | SEP3 | empty | − | − | − | − | − | − |
| BEL1 | SEP1 | AG | + + | / | + + | + + | + + | / |
| Empty | SEP1 | AG | + + | / | + | − | − | / |
| BEL1 | SEP1 | empty | − | / | − | − | − | / |
| BEL1 | SEP3 | SHP1 | + + | + + | + + | + | / | / |
| Empty | SEP3 | SHP1 | − | − | − | − | / | / |
| BEL1 | empty | SHP1 | − | − | − | − | / | / |
| BEL1 | SEP3 | SHP2 | + + | + + | + + | + | / | / |
| Empty | SEP3 | SHP2 | + | − | − | − | / | / |
| BEL1 | empty | SHP2 | − | − | − | − | / | / |
| BEL1 | SEP3 | STK | + + | + + | + | + | / | / |
| Empty | SEP3 | STK | + + | + | − | − | / | / |
| BEL1 | empty | STK | − | − | − | − | / | / |
| BEL1 | SEP3Δ192 | AG | / | / | − | − | / | / |
| Empty | SEP3Δ192 | AG | / | / | − | − | / | / |
| BEL1 | SEP3Δ192 | empty | / | / | − | − | / | / |
| BEL1Δ396 | SEP3 | AG | / | / | + + | + + | / | + + |
| BEL1Δ396 | empty | AG | / | / | − | − | / | − |
| BEL1Δ396 | SEP3 | empty | / | / | − | − | / | − |
−, no growth; +, growth on selective media; + +, strong growth, with formation of robust yeast colonies; /, not tested. 3AT, 3-aminotriazole; W, Trp; L, Leu; H, His; A, adenine. The significant interactions in normal style controls are in bold.