Figure 2.

Rice MEL1 Is Expressed Specifically in Reproductive Organs.

(A) RT-PCR analysis of MEL1 (top) and Actin (bottom) as a positive control in vegetative and reproductive organs. P1 to P5 indicate stages of panicle development; a P1 panicle is 5 mm long or less, P2 is ∼10 mm, P3 is ∼30 mm, P4 is ∼60 mm, and P5 is ∼100 mm.

(B) RT-PCR (15 cycles) products for four loci, MEL1, PAIR1, PAIR2, and Actin, were compared for wild-type (+/+) and mel1-1 mutant (−/−) anthers, respectively, at meiosis I for a mixture of 0.5- to 0.8-mm anthers. RT-PCR without reverse transcriptase (−) was performed as a negative control. The signal intensity ratio of the mel1-1 mutant signal to the wild-type signal, in which the ratio for the Actin locus was set as 1.0, is shown at right for each locus.