Figure 1.

Isolation of the hsr8-1 Mutant.

(A) Luciferase activity in the hsr8-1 mutant is higher than that in the wild type. A3L3 is the parental line of hsr8-1. hsr8-1com is hsr8-1 transformed with the genomic wild-type HSR8 sequence.

(B) Luciferase levels were measured in 9-d-old seedlings of the A3L3 parental line, hsr8-1, and hsr8-1com grown on Murashige and Skoog (MS) medium containing 1% glucose. Seedlings were sprayed with luciferin and analyzed luminometrically. Error bars represent se (n > 60).

(C) RT-PCR analysis of transcript levels in the hsr8-1 mutant. RT-PCR was performed on first-strand cDNA made from 9-d-old seedlings grown in constant light on medium containing 1% glucose. cDNA was standardized by reference to an actin standard.