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. 2007 Aug;19(8):2653–2661. doi: 10.1105/tpc.107.052597

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Copyright © 2007, American Society of Plant Biologists

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Figure 2. — Mapping of Disulfide Bonds in At GCL.

(A) ESI-Q-TOF mass spectrum (mass-to-charge ratio [m/z] of 400 to 1600) of an unfractionated tryptic digestion; the inset (m/z of 1260 to 1560) highlights the 6+ and 5+ charge states corresponding to the disulfide-containing peptide of interest (theoretical M_r: 7603.50 D).

(B) Tandem mass spectrometry (MS/MS) fragmentation of the 6+ charge state (m/z of 1268).

(C) Graphical fragment map correlating the fragmentation ions to the sequence of the disulfide-containing peptide. The disulfide-linked Cys residues are circled.

(D) ESI-Q-TOF mass spectrum (m/z of 400 to 1600) of an unfractionated tryptic digestion; the inset (m/z of 820 to 1075) highlights the 5+ and 4+ charge states corresponding to the disulfide-containing peptide of interest (theoretical M_r: 4245.80 D).

(E) MS/MS fragmentation of the 5+ charge state (m/z of 850).

(F) Graphical fragment map correlating the fragmentation ions to the sequence of the disulfide-containing peptide. The disulfide-linked Cys residues are circled.