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Isolation of mutants. FACS was used to isolate cells that express recombinant αIIbα6Aβ3β1 integrin, but in an inactive form. Cells were double-stained for expression of αIIbβ3 with D57 (y axis) and with the activation-specific antibody, PAC1 (x axis), as described. Rare cells that expressed the recombinant integrin, but failed to bind PAC1, were isolated (sort window).
