Table 1.
Percentage of total Aß comprised by Aß42(43) secreted from cells expressing wild-type or N141I mutant PS2 genes
| COS-1 Cells
|
Neuro2a Cells
|
||||
|---|---|---|---|---|---|
| cDNAs | %Aßx-42(43) | %Aß1-42(43) | Clone | %Aßx-42(43) | Average |
| Null | 16.2 | ND | Null.a | 10.2 | 10.2 |
| PS2 | ND | ND | Null.b | 10.1 | |
| PS2N141I | ND | ND | Vector.a | 11.9 | 11.5 |
| APP | 13.3 | 14.7 | Vector.b | 11.1 | |
| APP + PS2 | 14.9 | 19.9 | PS2.a | 11.6 | |
| APP + PS2N141I | 27.9 | 30.7 | PS2.b | 12.7 | 13.8 |
| APPNL | 8.7 | 6.8 | PS2.c | 17.0 | |
| APPNL + PS2 | 12.1 | 7.7 | PS2N141I.a | 57.0 | |
| APPNL + PS2N141I | 28.8 | 29.4 | PS2N141I.b | 39.0 | 52.0 |
| C100 | 10.4 | 15.4 | PS2N141I.c | 59.9 | |
| C100 + PS2 | 15.8 | 13.2 | |||
| C100 + PS2N141I | 34.6 | 38.5 | |||
Percentages of total Aß [= Aßx-40 + Aßx-42(43) or Aß1-40 + Aß1-42(43)] comprised by Aßx-42(43) or Aß1-42(43), respectively, are shown. Abbreviations for cDNAs transfected into COS-1 cells and the names of N2a clones are as in Fig. 3. The “+” means double transfection. Null, nontransfected; vector, transfected with empty pcDNA3 vector; ND, not detected.