Figure 5.

Single-channel currents, elicited by AcCho in an oocyte expressing muscle α₁β₁γδ nAcChoRs, recorded in the cell-attached configuration. Patch pipettes (≈10 MΩ) were filled with Ringer’s solution containing 500 nM AcCho alone (A), or AcCho plus 1 μM fluoxetine (B). The resting membrane potential of the oocyte was recorded with intracellular microelectrodes just before the single-channel recording (approximately −60 mV in this oocyte). The patch-pipette potential was +120 mV. Records were filtered at 2 kHz with a low-pass Bessel filter and digitized at 20 kHz.