Figure 7.

In situ localization of ω-3FAD mRNA in infected (arrows) and uninfected areas of parsley leaf buds. Two adjacent cross sections were hybridized with antisense (A) or sense (C) ω-3FAD RNA 6 h postinoculation with P. sojae spores. Infection sites (arrows) were detected by UV fluorescence of necrotic spots (B) in the same section as shown in A. Larger magnifications of selected areas in A are shown in D and E. Another cross-section through a separate leaf bud was taken 10 h postinoculation for UV light exposure (F) and hybridization with ω-3FAD antisense RNA (G). (Bars = 100 μm.)