Figure 2.

Ligand blot analysis of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate-extracted total brush border membrane vesicles proteins of first-instar YSB larvae. Equal amount of extracts in each lane were electrophoresed in an SDS/10% polyacrylamide gel and blotted onto nitrocellulose membrane; the membrane was cut into strips, and the strips were incubated in the respective toxins. The strips were probed further using anti-CryIA polyclonal rabbit IgG followed by anti-rabbit alkaline phosphatase (Sigma). Blots were processed using nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate mixture (Sigma). Lanes: 1, CryIAc; 2, CryIAb; 3, negative control (without toxin treatment).