Figure 1.

Diagram of MAT vector pNPI106. Plasmid pNPI106 has a “hit and run” cassette in which the chimeric ipt gene with ³⁵S promoter is inserted into Ac as a selectable marker. The gusA and nptII genes are unselected markers in these experiments. Arrows, PCR primers (see Fig. 3); 35S-P, CaMV ³⁵S promoter; ipt, isopentenyl transferase gene; T, isopentenyl transferase terminator; N-P, nopaline synthase promoter; N-T, nopaline synthase terminator; nptII, neomycin phosphotransferase gene; gusA, β-glucuronidase gene.