Figure 3.

Sequence analysis of cloned gfp mRNAs. (A) Autoradiograph and sequence of the amplified gfp mRNA sequence. Nucleotides 380–463 are absent from the transcribed sequence, and the site of this 84-nt deletion is indicated by an arrowhead. (B) Comparison of gfp cryptic intron sequences with the conserved sequences that are found at the splice sites and branchpoints of plant introns (21, 22). Splice sites are indicated by arrowheads, and the nucleotide involved in branchpoint formation is shown in reverse type.