Figure 3.

Fraction of sequences cloned in ORFs. Clones were picked at random from the different sized cDNA libraries that had been subjected to selection with 0, 12.5, 37.5, or 100 μg/ml kanamycin. Two independent methods, DNA sequencing and Western blotting, were used to determine whether they were cloned in ORFs. Black and gray bars represent groups of clones from libraries in E. coli DH10B and E. coli XL1-Blue, respectively. The numbers of clones analyzed in each of the four sets is shown in the corresponding bar.