Table 2.
Host | [kan], μg/ml | Library
|
||
---|---|---|---|---|
100- to 200-bp | 200- to 300-bp | 300- to 500-bp | ||
E. coli DH10B | 0 | 0.3 | 0 | 0 |
12.5 | 1 | 0.7 | 0.5 | |
37.5 | 1 | 0.7 | 0.7 | |
E. coli XL1-Blue | 100 | 0.7 | 0.7 | 0.8 |
The fraction of the scored matches (Table 1) that are in the reading frame actually translated by the host bacterium are shown. [kan], Kanamycin concentration.