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2D 15N–1H HMQC spectra of RNA I. (A) The free RNA in the absence of Mg2+. (B) The free RNA in the presence of 15 mM Mg2+. (C) The RNA complexed with protein S8. The peak marked X in A could not be definitively assigned as no NOE interactions were observed for this resonance, but it most likely corresponds to the imino resonance of U641.
