Figure 2.

Inactivation of V-CPO upon incubation with the phosphatase substrate p-NPP. V-CPO was incubated in 0.5 mM p-NPP at 25°C in 100 mM citrate (pH 5.0). At different time points samples were taken and CPO activity was measured by following the chlorination of monochlorodimedon (MCD, ɛ_{290 nm} = 20.2 mM⁻¹·cm⁻¹) into dichlorodimedon (ɛ_{290 nm} = 0.2 mM⁻¹·cm⁻¹). The assay contained 100 mM citrate (pH 5.0), 50 μM MCD, 1 mM H₂O₂, and 5 mM Cl⁻.