Figure 3.

Phosphatase activity of apo-r-CPO as a function of p-NPP concentration. apo-r-CPO (100 nM) was incubated with different concentrations of p-NPP at 25°C in 100 mM citrate (pH 5.0). After 6 h reaction mixtures were quenched with NaOH, and production of p-NP was measured at 410 nm using ɛ_{410 nm} = 18.3 mM⁻¹·cm⁻¹ at pH 12.