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Immunoprecipitation analysis of different recombinant pVHL complexes. pVHL complexes VHL-F, F-VHL, and F-VHL-157Δ were renatured in the presence or absence of recombinant elongin B-C followed by immunoprecipitation with M2 antibody and analysis on a SDS/14% polyacrylamide gel stained with Coomassie Blue (F, FLAG epitope). The bands at 55 and 30 kDa, present in all lanes, represent the heavy and light IgG chains, respectively.
