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Colocalization of Hs-CUL-2 and pVHL-GFP in transfected cells. COS-7 cells were transiently transfected with cDNAs encoding Hs-CUL-2-HA or pVHL-GFP (wt) separately (A) or concurrently (B–E). (C–E) COS-7 cells were transfected with cDNAs encoding Hs-CUL-2-HA and mutant pVHL-GFP [Δ60 (C and D) and 157Δ (E)]. Cells were stained with the anti-HA antibody to detect Hs-CUL-2-HA or observed under fluorescein isothiocyanate illumination to detect pVHL-GFP.
