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Heterologous assemblies containing a fusion protein. A 64-mer (Lower) carrying a recognition site for M·MspI and a recognition site for M·HhaI spaced at a distance of 30 bp was used. (Left) The 32P-end-labeled reaction products were separated by electrophoresis through polyacrylamide gel. (Right) The gel was transferred to polyvinylidene difluoride membrane, and the western blots were probed with anti-MRGShis6-antibodies to detect the M·HhaI-dod fusion protein.
