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Subcellular localization of PP-1Cα. KMls cells were synchronized at the G2/M phase, pelleted, and fractionated; W, S, and P indicate whole cell lysates, soluble, and particulate fractions, respectively. Aliquots (20 μg protein) were subjected to SDS/PAGE and immunoblotting with either G-97 antibody (Left) or anti-PP-1Cα antibody (Right). Identical results were obtained for the subcellular distribution of PP-1Cγ.
