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Dependence on superhelical density of the reactivity of promoter P2 nontemplate (Upper) and template (Lower) strands to different probes: CAA (Left), MBN (Center), and T7 endo I (Right). The sequences of the two strands (arrows indicate inverted repeats) are shown next to the gels. Short arrows, modification or cleavage sites; σ̄ is superhelical density.
